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KMID : 0383919710080000193
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Report of National Institute of Health
1971 Volume.8 No. 0 p.193 ~ p.201
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Isolative Determination of dl-αTocopherol and d-Biotin in the Multivitamins
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李海彬/Lee, H.B.
高旺鎭/朴茂鎭/沈壽一/Ko, W.C./Park, M.C./Shim, S.L.
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Abstract
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dl α-Tocopherol in the multivitamins was saponified and seperated with thin layer chromatography.
It was colored by Ferric chloride and α, α´-Dipyridyl.
dl-α-Tocopherol and d-Boitin in ithe multivitamins could be determined with thin layer chromatography and ncolorimetry used by Emerie-Engel method to dl--Tocopherol and p-DACA methoed to d-Biotin.
The results summerized as follows:
1. Same maximum absworbance are given at 520mu to dl-α-Tocopherol and using the above muntioned method.
2. Beer´s law obeyed for 30~160mcg/ml to dl-α-Tocopherol and 30~140 mcg/ml to d-Biotin.
3. Reproducibility is good samely for two components, that is, offered S≤4.3% to dl-α-Tocopherol and S≤3.0% to d-Biotin.
4. Thin layer chromatogoraphic and spectrophotometric have been developed for rapid and fairly accurate determination of dl-α-Tocopherol and d-Biotin in the multivitamins.
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